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his60 ni957 superflow resin  (TaKaRa)


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    TaKaRa his60 ni957 superflow resin
    His60 Ni957 Superflow Resin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/his60 ni957 superflow resin/product/TaKaRa
    Average 96 stars, based on 19 article reviews
    his60 ni957 superflow resin - by Bioz Stars, 2026-03
    96/100 stars

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    Recommended workflow for ACSA2-based astrocyte isolation with reduced microglial contamination. Adult brain tissue is enzymatically dissociated with the Miltenyi Adult Brain Dissociation kit (P), followed by myelin bead removal to remove myelin debris and reduce microglial contamination. Single-cell suspensions are stained for ACSA2, CD45, and CD11b, then sorted by FACS. Gating on ACSA2 + /CD45/CD11b cells excludes residual microglia and yields higher purity astrocyte populations. Created in BioRender. Daniele, E. (2026) https://BioRender.com/t52lw3k .

    Journal: Frontiers in Cellular Neuroscience

    Article Title: ACSA2 is not astrocyte-specific: implications for cell sorting strategies in the rodent brain

    doi: 10.3389/fncel.2026.1756677

    Figure Lengend Snippet: Recommended workflow for ACSA2-based astrocyte isolation with reduced microglial contamination. Adult brain tissue is enzymatically dissociated with the Miltenyi Adult Brain Dissociation kit (P), followed by myelin bead removal to remove myelin debris and reduce microglial contamination. Single-cell suspensions are stained for ACSA2, CD45, and CD11b, then sorted by FACS. Gating on ACSA2 + /CD45/CD11b cells excludes residual microglia and yields higher purity astrocyte populations. Created in BioRender. Daniele, E. (2026) https://BioRender.com/t52lw3k .

    Article Snippet: The olfactory bulb and cerebellum were removed, and brain tissue was washed in calcium/magnesium-free DPBS (Wisent, MultiCell, cat# 311-425), prior to dissociation with the Adult Brain Dissociation Kit (P) kit (Miltenyi Biotec, cat# 130-107-677).

    Techniques: Isolation, Single Cell, Staining